Chapter title |
Virus-Mediated Expression of DREADDs for In Vivo Metabolic Studies
|
---|---|
Chapter number | 14 |
Book title |
G Protein-Coupled Receptors in Drug Discovery
|
Published in |
Methods in molecular biology, January 2015
|
DOI | 10.1007/978-1-4939-2914-6_14 |
Pubmed ID | |
Book ISBNs |
978-1-4939-2913-9, 978-1-4939-2914-6
|
Authors |
Mario Rossi, Zhenzhong Cui, Ken-ichiro Nakajima, Jianxin Hu, Lu Zhu, Jürgen Wess, Rossi, Mario, Cui, Zhenzhong, Nakajima, Ken-ichiro, Hu, Jianxin, Zhu, Lu, Wess, Jürgen |
Abstract |
During the past few years, CNO-sensitive designer G protein-coupled receptors (GPCRs) known as DREADDs (designer receptors exclusively activated by designer drugs) have emerged as powerful new tools for the study of GPCR physiology. In this chapter, we present protocols employing adeno-associated viruses (AAVs) to express a Gq-coupled DREADD (Dq) in two metabolically important cell types, AgRP neurons of the hypothalamus and hepatocytes of the liver. We also provide examples dealing with the metabolic analysis of the Dq mutant mice after administration of CNO in vivo. The approaches described in this chapter can be applied to other members of the DREADD family and, of course, different cell types. It is likely that the use of DREADD technology will identify physiologically important signaling pathways that can be targeted for therapeutic purposes. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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United Kingdom | 1 | 7% |
Unknown | 13 | 93% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 3 | 21% |
Student > Doctoral Student | 2 | 14% |
Unspecified | 1 | 7% |
Other | 1 | 7% |
Student > Bachelor | 1 | 7% |
Other | 1 | 7% |
Unknown | 5 | 36% |
Readers by discipline | Count | As % |
---|---|---|
Neuroscience | 5 | 36% |
Biochemistry, Genetics and Molecular Biology | 2 | 14% |
Agricultural and Biological Sciences | 1 | 7% |
Unspecified | 1 | 7% |
Unknown | 5 | 36% |