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Mendeley readers
| Chapter title |
Single-Molecule Fluorescence Microscopy for the Analysis of Fast Receptor Dynamics
|
|---|---|
| Chapter number | 5 |
| Book title |
G Protein-Coupled Receptors in Drug Discovery
|
| Published in |
Methods in molecular biology, January 2015
|
| DOI | 10.1007/978-1-4939-2914-6_5 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-2913-9, 978-1-4939-2914-6
|
| Authors |
Julia Wagner, Titiwat Sungkaworn, Katrin G. Heinze, Martin J. Lohse, Davide Calebiro, Wagner, Julia, Sungkaworn, Titiwat, Heinze, Katrin G., Lohse, Martin J., Calebiro, Davide |
| Abstract |
Assessing the dynamics of individual membrane proteins in living cells is a powerful approach to investigate their assembly, mobility, and function. Here, we describe how to image single G protein-coupled receptors (GPCRs), both in the active and inactive state. This is achieved by combining labeling of GPCRs with bright organic fluorophores and fluorescent imaging by total internal reflection fluorescence microscopy. Using this method, individual tracks of single molecules can be analyzed in parallel with high spatial precision and with frame rates up to 50/s. |
Mendeley readers
The data shown below were compiled from readership statistics for 20 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 20 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 4 | 20% |
| Researcher | 3 | 15% |
| Student > Bachelor | 2 | 10% |
| Student > Master | 2 | 10% |
| Professor | 1 | 5% |
| Other | 2 | 10% |
| Unknown | 6 | 30% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 6 | 30% |
| Agricultural and Biological Sciences | 3 | 15% |
| Pharmacology, Toxicology and Pharmaceutical Science | 2 | 10% |
| Medicine and Dentistry | 1 | 5% |
| Neuroscience | 1 | 5% |
| Other | 1 | 5% |
| Unknown | 6 | 30% |