| Chapter title |
Bioluminescence Resonance Energy Transfer Approaches to Discover Bias in GPCR Signaling
|
|---|---|
| Chapter number | 13 |
| Book title |
G Protein-Coupled Receptors in Drug Discovery
|
| Published in |
Methods in molecular biology, January 2015
|
| DOI | 10.1007/978-1-4939-2914-6_13 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-2913-9, 978-1-4939-2914-6
|
| Authors |
Elizabeth K. M. Johnstone, Kevin D. G. Pfleger, Johnstone, Elizabeth K. M., Pfleger, Kevin D. G. |
| Abstract |
Bioluminescence resonance energy transfer (BRET) is a well-established technique for investigating G protein-coupled receptor (GPCR) pharmacology. BRET enables the monitoring of molecular proximity through the use of heterologously expressed proteins of interest and/or fluorophore-labeled ligands. Fusion to a donor luciferase enzyme or an acceptor fluorophore and subsequent detection of resonance energy transfer indicate the close proximity of the molecules of interest. As BRET is readily applied to the study of numerous GPCR signaling and regulatory paths, it is an ideal technique for investigating the pharmacology of biased ligands and receptors. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Czechia | 1 | 8% |
| Unknown | 11 | 92% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 4 | 33% |
| Student > Master | 3 | 25% |
| Researcher | 2 | 17% |
| Student > Bachelor | 1 | 8% |
| Unknown | 2 | 17% |
| Readers by discipline | Count | As % |
|---|---|---|
| Pharmacology, Toxicology and Pharmaceutical Science | 4 | 33% |
| Biochemistry, Genetics and Molecular Biology | 4 | 33% |
| Linguistics | 1 | 8% |
| Unknown | 3 | 25% |