| Chapter title |
Radioligand Binding Assay for an Exon 11-Associated Mu Opioid Receptor Target
|
|---|---|
| Chapter number | 16 |
| Book title |
G Protein-Coupled Receptors in Drug Discovery
|
| Published in |
Methods in molecular biology, January 2015
|
| DOI | 10.1007/978-1-4939-2914-6_16 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-2913-9, 978-1-4939-2914-6
|
| Authors |
Gina F. Marrone, Susruta Majumdar, Gavril W. Pasternak, Marrone, Gina F., Majumdar, Susruta, Pasternak, Gavril W. |
| Abstract |
Receptor binding provides a valuable approach for characterization of drugs and their receptors. There are three major families of opioid receptors: mu, delta, and kappa. Highly selective radioligands are available for all three classes of traditional receptors. Of the three, the mu receptor undergoes extensive alternative splicing, generating a number of traditional mu receptor subtypes as well as a nontraditional, truncated set of variants associated with exon 11. These exon 11-associated truncated variants are not readily labeled with current radioligands. Here we describe the synthesis of a radioiodinated ligand suitable for carrying out binding studies for this target. |
X Demographics
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 4 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Professor | 1 | 25% |
| Student > Bachelor | 1 | 25% |
| Student > Postgraduate | 1 | 25% |
| Unknown | 1 | 25% |
| Readers by discipline | Count | As % |
|---|---|---|
| Pharmacology, Toxicology and Pharmaceutical Science | 2 | 50% |
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