Chapter title |
Poly( N,N -Dimethylacrylamide)-Based Coatings to Modulate Electroosmotic Flow and Capillary Surface Properties for Protein Analysis
|
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Chapter number | 9 |
Book title |
Capillary Electrophoresis of Proteins and Peptides
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-4014-1_9 |
Pubmed ID | |
Book ISBNs |
978-1-4939-4012-7, 978-1-4939-4014-1
|
Authors |
Laura Sola, Marina Cretich, Marcella Chiari |
Abstract |
Capillary electrophoresis (CE) is one of the most powerful techniques for the separation of biomolecules. However, the separation efficiency of proteins in CE is often compromised by their tendency to interact with the silanol groups on the surface of the inner capillary and by an uncontrolled electroosmotic flow. Herein, we report on the use of novel hydrophilic polymeric coatings that can modulate the properties of the capillary walls. The novelty of these poly(N,N-dimethylacrylamide)-based copolymers relies on the simultaneous presence of chemically reactive groups (N-acryloyloxysuccinimide and glycidyl methacrylate) and silane groups in the backbone, which results in highly stable films due to the covalent reaction between the polymer and the glass silanols. A careful optimization of monomer concentration confers anti-fouling properties to the polymer coatings, and thus allows for highly efficient acidic and alkaline protein separations. Furthermore, the presence of these monomers makes it possible to modulate the electroosmotic flow from negligible to reduced values, depending on the desired application. |
Mendeley readers
Geographical breakdown
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Unknown | 7 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Researcher | 3 | 43% |
Student > Ph. D. Student | 1 | 14% |
Student > Doctoral Student | 1 | 14% |
Unknown | 2 | 29% |
Readers by discipline | Count | As % |
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Chemistry | 3 | 43% |
Pharmacology, Toxicology and Pharmaceutical Science | 1 | 14% |
Unknown | 3 | 43% |