Chapter title |
Analysis of Somatropin by Double-Injection Capillary-Zone Electrophoresis in Polybrene/Chondroitin Sulfate A Double-Coated Capillaries
|
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Chapter number | 8 |
Book title |
Capillary Electrophoresis of Proteins and Peptides
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-4014-1_8 |
Pubmed ID | |
Book ISBNs |
978-1-4939-4012-7, 978-1-4939-4014-1
|
Authors |
Ahmad Amini |
Abstract |
Purity determination of somatropin as a recombinant protein is important to ensure its safety and quality. This is carried out by capillary zone electrophoresis in double-injection mode using polybrene/chondroitin sulfate A double-coated capillaries. Modification of the capillary wall eliminates protein-wall interactions which results in improved accuracy and precision of the determinations. In the double-injection mode two somatropin samples are analyzed within a single electrophoretic run. Prior to the second injection, the first injected plug is electrophoresed for a predetermined time period in order to adjust the inter-plug distance. Here, the principle for the separation of somatropin charge variants is described. |
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