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Chapter title |
On-Line Solid-Phase Extraction Capillary Electrophoresis Mass Spectrometry for Preconcentration and Clean-Up of Peptides and Proteins
|
---|---|
Chapter number | 6 |
Book title |
Capillary Electrophoresis of Proteins and Peptides
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-4014-1_6 |
Pubmed ID | |
Book ISBNs |
978-1-4939-4012-7, 978-1-4939-4014-1
|
Authors |
Fernando Benavente, Silvia Medina-Casanellas, Estela Giménez, Victoria Sanz-Nebot |
Abstract |
One of the major drawbacks of capillary electrophoresis (CE) and other microscale separation techniques, for the analysis of low abundant peptides and proteins in complex samples, are the poor concentration limits of detection. Several strategies have been developed to improve CE sensitivity. Here, we describe an on-line solid-phase extraction capillary electrophoresis mass spectrometry method with a commercial C18 sorbent for clean-up and preconcentration of neuropeptides from highly diluted biological samples. |
Mendeley readers
The data shown below were compiled from readership statistics for 17 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 17 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 5 | 29% |
Student > Master | 3 | 18% |
Unspecified | 2 | 12% |
Professor | 2 | 12% |
Student > Doctoral Student | 1 | 6% |
Other | 3 | 18% |
Unknown | 1 | 6% |
Readers by discipline | Count | As % |
---|---|---|
Chemistry | 9 | 53% |
Unspecified | 2 | 12% |
Pharmacology, Toxicology and Pharmaceutical Science | 2 | 12% |
Linguistics | 1 | 6% |
Nursing and Health Professions | 1 | 6% |
Other | 1 | 6% |
Unknown | 1 | 6% |