Chapter title |
Screening of Small Intact Proteins by Capillary Electrophoresis Electrospray Ionization-Mass Spectrometry (CE-ESI-MS)
|
---|---|
Chapter number | 4 |
Book title |
Capillary Electrophoresis of Proteins and Peptides
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-4014-1_4 |
Pubmed ID | |
Book ISBNs |
978-1-4939-4012-7, 978-1-4939-4014-1
|
Authors |
Sabine Neuberger, Angelina Rafai, Christian Neusüß |
Abstract |
Capillary electrophoresis (CE) has been shown to be a suitable separation technique for complex samples. Combined with electrospray ionization-mass spectrometry (ESI-MS), it is a powerful tool offering the opportunity of high selectivity and sensitivity combined with the possibility to identify and characterize intact proteins. In this protocol, we demonstrate a screening method for intact proteins based on capillary zone electrophoresis (CZE) separation coupled with online mass spectrometric detection. In order to avoid protein-wall interactions, a neutral coated capillary is used to create a universal method for proteins with both low and high electrophoretic mobilities. In addition, we show the successful validation and application of this screening method for a set of eight standard proteins and the glycoprotein erythropoietin. |
Mendeley readers
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Demographic breakdown
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Student > Ph. D. Student | 2 | 22% |
Student > Doctoral Student | 1 | 11% |
Researcher | 1 | 11% |
Unknown | 2 | 22% |
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Unknown | 4 | 44% |