Chapter title |
Capillary Electrophoresis-Ultraviolet-Mass Spectrometry (CE-UV-MS) for the Simultaneous Determination and Quantification of Insulin Formulations
|
---|---|
Chapter number | 15 |
Book title |
Capillary Electrophoresis of Proteins and Peptides
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-4014-1_15 |
Pubmed ID | |
Book ISBNs |
978-1-4939-4012-7, 978-1-4939-4014-1
|
Authors |
Julie Schappler, Serge Rudaz |
Abstract |
This chapter describes a CE-UV-MS method for the identification and quantification of insulin in pharmaceutical formulations in a single run. The CE conditions are optimized to avoid the adsorption of the protein onto the capillary wall. Particular attention is paid regarding the choice of the internal standard. A strategy based on multiple injections is applied to correct both ionization and injection variabilities. The methodology is validated according to international guidelines and the obtained accuracy profile demonstrates the ability of the CE-UV-MS method to quantify insulin in pharmaceutical formulations within a ±5 % acceptance range. This strategy can be implemented in the field of quality control, as well as in the detection of counterfeits. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 8 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 3 | 38% |
Unspecified | 1 | 13% |
Student > Doctoral Student | 1 | 13% |
Other | 1 | 13% |
Student > Master | 1 | 13% |
Other | 1 | 13% |
Readers by discipline | Count | As % |
---|---|---|
Pharmacology, Toxicology and Pharmaceutical Science | 3 | 38% |
Unspecified | 1 | 13% |
Biochemistry, Genetics and Molecular Biology | 1 | 13% |
Agricultural and Biological Sciences | 1 | 13% |
Chemistry | 1 | 13% |
Other | 0 | 0% |
Unknown | 1 | 13% |