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Date Palm Biotechnology Protocols Volume I

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Cover of 'Date Palm Biotechnology Protocols Volume I'

Table of Contents

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    Book Overview
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    Chapter 1 Cultivar-Dependent Direct Organogenesis of Date Palm from Shoot Tip Explants
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    Chapter 2 NAA-Induced Direct Organogenesis from Female Immature Inflorescence Explants of Date Palm
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    Chapter 3 Direct Organogenesis from Immature Female Inflorescence of Date Palm by Gradual Reduction of 2,4-D Concentration
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    Chapter 4 Optimized Direct Organogenesis from Shoot Tip Explants of Date Palm
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    Chapter 5 Direct Organogenesis and Indirect Somatic Embryogenesis by In Vitro Reversion of Mature Female Floral Buds to a Vegetative State
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    Chapter 6 Enhanced Indirect Somatic Embryogenesis of Date Palm Using Low Levels of Seawater
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    Chapter 7 Enhanced Indirect Somatic Embryogenesis from Shoot-Tip Explants of Date Palm by Gradual Reductions of 2,4-D Concentration
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    Chapter 8 Indirect Somatic Embryogenesis from Mature Inflorescence Explants of Date Palm
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    Chapter 9 Indirect Somatic Embryogenesis of Date Palm Using Juvenile Leaf Explants and Low 2,4-D Concentration
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    Chapter 10 Desiccation-Enhanced Maturation and Germination of Date Palm Somatic Embryos Derived from Cell Suspension Culture
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    Chapter 11 Desiccation and Cold Hardening of Date Palm Somatic Embryos Improve Germination
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    Chapter 12 Histological Evidence of Indirect Somatic Embryogenesis from Immature Female Date Palm Inflorescences
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    Chapter 13 Histological Analysis of the Developmental Stages of Direct Somatic Embryogenesis Induced from In Vitro Leaf Explants of Date Palm
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    Chapter 14 Identifying and Controlling Contamination of Date Palm Tissue Cultures
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    Chapter 15 Controlling Hyperhydricity in Date Palm In Vitro Culture by Reduced Concentration of Nitrate Nutrients
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    Chapter 16 Improvement of In Vitro Date Palm Plantlet Acclimatization Rate with Kinetin and Hoagland Solution
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    Chapter 17 Plant Regeneration from Somatic Embryogenic Suspension Cultures of Date Palm
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    Chapter 18 Synchronization of Somatic Embryogenesis in Date Palm Suspension Culture Using Abscisic Acid
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    Chapter 19 Microcalli Induction in Protoplasts Isolated from Embryogenic Callus of Date Palm
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    Chapter 20 Temporary Immersion System for Date Palm Micropropagation
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    Chapter 21 Plantform Bioreactor for Mass Micropropagation of Date Palm
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    Chapter 22 Genetic Transformation of Date Palm Via Microprojectile Bombardment
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    Chapter 23 Microprojectile Bombardment Transformation of Date Palm Using the Insecticidal Cholesterol Oxidase ( ChoA ) Gene
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    Chapter 24 Transient GUS Gene Expression in Date Palm Fruit Using Agroinjection Transformation Technique
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    Chapter 25 Bioreactor Steroid Production and Analysis of Date Palm Embryogenic Callus
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    Chapter 26 Extraction and Estimation of Secondary Metabolites from Date Palm Cell Suspension Cultures
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    Chapter 27 In Vitro Assessment of Abiotic Stress in Date Palm: Salinity and Drought
Attention for Chapter 18: Synchronization of Somatic Embryogenesis in Date Palm Suspension Culture Using Abscisic Acid
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Chapter title
Synchronization of Somatic Embryogenesis in Date Palm Suspension Culture Using Abscisic Acid
Chapter number 18
Book title
Date Palm Biotechnology Protocols Volume I
Published in
Methods in molecular biology, January 2017
DOI 10.1007/978-1-4939-7156-5_18
Pubmed ID
Book ISBNs
978-1-4939-7155-8, 978-1-4939-7156-5
Authors

Alwael, Hussain A., Naik, Poornananda M., Al-Khayri, Jameel M., Hussain A. Alwael, Poornananda M. Naik, Jameel M. Al-Khayri

Abstract

Somatic embryogenesis is considered the most effective method for commercial propagation of date palm. However, the limitation of obtaining synchronized development of somatic embryos remains an impediment. The synchronization of somatic embryo development is ideal for the applications to produce artificial seeds. Abscisic acid (ABA) is associated with stress response and influences in vitro growth and development. This chapter describes an effective method to achieve synchronized development of somatic embryos in date palm cell suspension culture. Among the ABA concentrations tested (0, 1, 10, 50, 100 μM), the best synchronized growth was obtained in response to 50-100 μM. Here we provide a comprehensive protocol for in vitro plant regeneration of date palm starting with shoot-tip explant, callus initiation and growth, cell suspension establishment, embryogenesis synchronization with ABA treatment, somatic embryo germination, and rooting as well as acclimatized plantlet establishment.

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The data shown below were compiled from readership statistics for 10 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 10 100%

Demographic breakdown

Readers by professional status Count As %
Student > Master 3 30%
Professor 2 20%
Librarian 1 10%
Student > Ph. D. Student 1 10%
Researcher 1 10%
Other 0 0%
Unknown 2 20%
Readers by discipline Count As %
Agricultural and Biological Sciences 4 40%
Chemical Engineering 1 10%
Engineering 1 10%
Unknown 4 40%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 28 April 2018.
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#15,506,823
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Outputs from Methods in molecular biology
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Outputs of similar age from Methods in molecular biology
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