Chapter title |
Temporary Immersion System for Date Palm Micropropagation
|
---|---|
Chapter number | 20 |
Book title |
Date Palm Biotechnology Protocols Volume I
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-7156-5_20 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7155-8, 978-1-4939-7156-5
|
Authors |
Ahmed Othmani, Chokri Bayoudh, Amel Sellemi, Noureddine Drira, Othmani, Ahmed, Bayoudh, Chokri, Sellemi, Amel, Drira, Noureddine |
Abstract |
The temporary immersion system (TIS) is being used with tremendous success for automation of micropropagation of many plant species. TIS usually consists of a culture vessel comprising two compartments, an upper one with the plant material and a lower one with the liquid culture medium and an automated air pump. The latter enables contact between all parts of the explants and the liquid medium by setting overpressure to the lower part of the container. These systems are providing the most satisfactory conditions for date palm regeneration via shoot organogenesis and allow a significant increase of multiplication rate (5.5-fold in comparison with that regenerated on agar-solidified medium) and plant material quality, thereby reducing production cost. |
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