Chapter title |
Direct Organogenesis and Indirect Somatic Embryogenesis by In Vitro Reversion of Mature Female Floral Buds to a Vegetative State
|
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Chapter number | 5 |
Book title |
Date Palm Biotechnology Protocols Volume I
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-7156-5_5 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7155-8, 978-1-4939-7156-5
|
Authors |
Eman M. M. Zayed |
Abstract |
This protocol describes in vitro plant regeneration from mature female inflorescence explants of date palm (Phoenix dactylifera L.) by reversion of floral state (reproductive phase) to the vegetative state. The mature female inflorescence (fully developed) is cultured on MS induction medium containing 10 mg/L 2,4-dichlorophenoxyacetic acid (2,4-D), 3 mg/L 2-isopentenyladenine (2iP), and 2 mg/L paclobutrazol (PBZ) or 2 mg/L abscisic acid (ABA). The basal part of the petals has meristematic cells, which can be induced to initiate callus or direct shoot formation depending on the plant growth regulator amendments. Callus forms on the induction medium supplemented with PBZ after 12 weeks, whereas it differentiates into somatic embryos on a medium containing 0.1 mg/L naphthaleneacetic acid (NAA). Direct shoots are regenerated on the induction medium amended with ABA after 24 weeks. Procedures for plant regeneration from mature female inflorescence explants are described, and histological changes which occur during the reversion process are presented. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 10 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 3 | 30% |
Student > Master | 3 | 30% |
Researcher | 1 | 10% |
Unknown | 3 | 30% |
Readers by discipline | Count | As % |
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Agricultural and Biological Sciences | 4 | 40% |
Nursing and Health Professions | 1 | 10% |
Chemical Engineering | 1 | 10% |
Unknown | 4 | 40% |