| Chapter title |
Microprojectile Bombardment Transformation of Date Palm Using the Insecticidal Cholesterol Oxidase ( ChoA ) Gene
|
|---|---|
| Chapter number | 23 |
| Book title |
Date Palm Biotechnology Protocols Volume I
|
| Published in |
Methods in molecular biology, January 2017
|
| DOI | 10.1007/978-1-4939-7156-5_23 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-7155-8, 978-1-4939-7156-5
|
| Authors |
Mai A. Allam, Mahmoud M. Saker, Allam, Mai A., Saker, Mahmoud M. |
| Abstract |
The overall objective of this work is to optimize the transformation system for date palm as a first step toward production of date palm clones resistant to noxious pests. A construct harboring the cholesterol oxidase (ChoA) gene, which renders plant resistance against insect attack, is introduced into embryogenic date palm callus using the PDS-1000/He particle bombardment system. The process involves the establishment of embryogenic callus cultures as well as immature embryo-derived microcalli that are used as target tissues for shooting and optimization of transformation conditions. This chapter in addition explains molecular and histochemical assays conducted to confirm gene integration and expression. |
X Demographics
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Japan | 1 | 50% |
| Unknown | 1 | 50% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 2 | 100% |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 10 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 5 | 50% |
| Other | 2 | 20% |
| Professor | 1 | 10% |
| Researcher | 1 | 10% |
| Unknown | 1 | 10% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 3 | 30% |
| Agricultural and Biological Sciences | 3 | 30% |
| Nursing and Health Professions | 2 | 20% |
| Medicine and Dentistry | 1 | 10% |
| Unknown | 1 | 10% |