Chapter title |
Bioreactor Steroid Production and Analysis of Date Palm Embryogenic Callus
|
---|---|
Chapter number | 25 |
Book title |
Date Palm Biotechnology Protocols Volume I
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-7156-5_25 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7155-8, 978-1-4939-7156-5
|
Authors |
Sherif El-Sharabasy, Maiada El-Dawayati, El-Sharabasy, Sherif, El-Dawayati, Maiada |
Abstract |
Several compounds and families of compounds of date palm secondary metabolites have been investigated. The analysis of date palm tissue has shown the abundance of secondary metabolites including phytosterols, e.g., steroids, an important group of pharmaceutical compounds. Biotechnology offers the opportunity to utilize cells, tissues, and organs grown in vitro and manipulated to obtain desired compounds. This chapter presents a protocol for the production, determination, and identification of steroids in date palm callus tissue. The addition of 0.01 mg/L pyruvic acid as a precursor to MS liquid culture medium enhances steroid production. In addition, the chapter describes the sterol analytical techniques based on gas-liquid chromatography and gas chromatography-mass spectrometry. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 3 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 1 | 33% |
Professor > Associate Professor | 1 | 33% |
Student > Master | 1 | 33% |
Readers by discipline | Count | As % |
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Agricultural and Biological Sciences | 2 | 67% |
Unknown | 1 | 33% |