| Chapter title |
Controlling Hyperhydricity in Date Palm In Vitro Culture by Reduced Concentration of Nitrate Nutrients
|
|---|---|
| Chapter number | 15 |
| Book title |
Date Palm Biotechnology Protocols Volume I
|
| Published in |
Methods in molecular biology, January 2017
|
| DOI | 10.1007/978-1-4939-7156-5_15 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-7155-8, 978-1-4939-7156-5
|
| Authors |
Maiada M. El-Dawayati, Zeinab E. Zayed, El-Dawayati, Maiada M., Zayed, Zeinab E. |
| Abstract |
Hyperhydricity (or vitrification) is a fundamental physiological disorder in date palm micropropagation. Several factors have been ascribed as being responsible for hyperhydricity, which are related to the explant, medium, culture vessel, and environment. The optimization of inorganic nutrients in the culture medium improves in vitro growth and morphogenesis, in addition to controlling hyperhydricity. This chapter describes a protocol for controlling hyperhydricity during the embryogenic callus stage by optimizing the ratio of nitrogen salts of the Murashige and Skoog (MS) nutrient culture medium. The best results of differentiation from cured hyperhydric callus are obtained using modification at a ratio of NH(4+)/NO(3-) at 10:15 (825:1425 mg/L) of the MS culture medium to remedy hyperhydric date palm callus and achieve the recovery of normal embryogenic callus and subsequent regeneration of plantlets. Based on the results of this study, nutrient medium composition has an important role in avoiding hyperhydricity problems during date palm micropropagation. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 6 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Unspecified | 1 | 17% |
| Librarian | 1 | 17% |
| Student > Ph. D. Student | 1 | 17% |
| Professor > Associate Professor | 1 | 17% |
| Student > Master | 1 | 17% |
| Other | 0 | 0% |
| Unknown | 1 | 17% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 3 | 50% |
| Unspecified | 1 | 17% |
| Unknown | 2 | 33% |