Chapter title |
Recreating Intestinal Peristalsis in the Petri Dish
|
---|---|
Chapter number | 34 |
Book title |
Neurotrophic Factors
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-7571-6_34 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7570-9, 978-1-4939-7571-6
|
Authors |
Rosa Espinosa-Luna, Alma R. Barajas-Espinosa, Fernando Ochoa-Cortez, Carlos Barajas-López, Espinosa-Luna, Rosa, Barajas-Espinosa, Alma R., Ochoa-Cortez, Fernando, Barajas-López, Carlos |
Abstract |
Here we describe a culture technique of cells dissociated from the external muscularis of the guinea pig small intestine, which allows us to maintain all the elements involved in the intestinal peristaltic reflex. After a few days in culture, these cells reorganize to form a small group of cells that permit the generation of pacemaker activity, spontaneous contractions, and the development of inhibitory and excitatory junction potentials in the petri dish, all elements involved in the peristaltic reflex. Therefore, these co-cultures are suitable to study the cellular and molecular aspects related to the development, maintenance, and modulation of motor intestinal functions. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 6 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Professor | 1 | 17% |
Student > Bachelor | 1 | 17% |
Student > Master | 1 | 17% |
Unknown | 3 | 50% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 2 | 33% |
Nursing and Health Professions | 1 | 17% |
Unknown | 3 | 50% |