Chapter title |
Isolation and Culture of Brain Microvascular Endothelial Cells for In Vitro Blood-Brain Barrier Studies
|
---|---|
Chapter number | 23 |
Book title |
Neurotrophic Factors
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-7571-6_23 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7570-9, 978-1-4939-7571-6
|
Authors |
Hector Rosas-Hernandez, Elvis Cuevas, Susan M. Lantz, Merle G. Paule, Syed F. Ali, Rosas-Hernandez, Hector, Cuevas, Elvis, Lantz, Susan M., Paule, Merle G., Ali, Syed F. |
Abstract |
The blood-brain barrier (BBB) is essential to maintain the proper microenvironment for brain function. Although formed by different cell types, the endothelial cells (ECs) of the brain microvessels provide the BBB with its selective permeability. To study the BBB in vitro, EC lines as well as primary isolated ECs have been used. In this chapter, we will provide a detailed protocol on how to isolate and culture primary brain microvascular endothelial cells from different species for use as in vitro models of the BBB. When performed properly, this protocol will allow one to obtain a pure culture of brain microvascular endothelial cells with which to analyze the effects of therapeutic and toxic agents on BBB functions. |
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France | 1 | 25% |
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Demographic breakdown
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Scientists | 1 | 25% |
Mendeley readers
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Student > Master | 4 | 16% |
Other | 2 | 8% |
Researcher | 2 | 8% |
Lecturer > Senior Lecturer | 1 | 4% |
Other | 4 | 16% |
Unknown | 6 | 24% |
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Pharmacology, Toxicology and Pharmaceutical Science | 1 | 4% |
Other | 4 | 16% |
Unknown | 8 | 32% |