Chapter title |
Nerve Growth Factor-Induced Angiogenesis: 2. The Quail Chorioallantoic Membrane Assay
|
---|---|
Chapter number | 19 |
Book title |
Neurotrophic Factors
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-7571-6_19 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7570-9, 978-1-4939-7571-6
|
Authors |
Philip Lazarovici, Adi Lahiani, Galit Gincberg, Dikla Haham, Cezary Marcinkiewicz, Peter I. Lelkes, Lazarovici, Philip, Lahiani, Adi, Gincberg, Galit, Haham, Dikla, Marcinkiewicz, Cezary, Lelkes, Peter I. |
Abstract |
The avian chorioallantoic membrane (CAM) is a simple, highly vascularized extraembryonic membrane, which performs multiple functions during embryonic development. Therefore, the models of chicken and quail assays represent robust experimental platforms to study angiogenesis, which reflects perturbation of the entire vascular tree. This experimental approach, when combined with fractal morphometry, is sensitive to changes in vascular branching pattern and density. Nerve growth factor is a neurotrophin promoting angiogenesis in CAM models. Here, we provide a detailed protocol of the quail CAM, shell-less model, to study nerve growth factor effects on blood capillary sprouting. The quail CAM assay may be beneficial in investigations of cellular and molecular aspects of neurotrophin-induced angiogenesis and for developing novel anti-angiogenesis and anticancer therapies. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 9 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 2 | 22% |
Professor | 1 | 11% |
Other | 1 | 11% |
Student > Master | 1 | 11% |
Unknown | 4 | 44% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 2 | 22% |
Nursing and Health Professions | 1 | 11% |
Materials Science | 1 | 11% |
Medicine and Dentistry | 1 | 11% |
Unknown | 4 | 44% |