Chapter title |
Primary Motor Neuron Culture to Promote Cellular Viability and Myelination
|
---|---|
Chapter number | 32 |
Book title |
Neurotrophic Factors
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-7571-6_32 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7570-9, 978-1-4939-7571-6
|
Authors |
Jun-Kyo Francis Suh, Sujin Hyung, Suh, Jun-Kyo Francis, Hyung, Sujin |
Abstract |
A culture system that can recapitulate myelination in vitro will not only help us to better understand the mechanism of myelination and demyelination but also identify possible therapeutic interventions for treating demyelinating diseases. Here, we introduce a simple and reproducible myelination culture system using mouse motor neurons (MNs) and Schwann cells (SCs). Dissociated motor neurons are plated on a feeder layer of SCs, which interact with and wrap around the axons of MNs as they differentiate in culture. In our MN-SC co-culture system, MNs survive over 3 weeks and extend long axons. Both viability and axon growth of MNs in the co-culture are markedly enhanced as compared to those of MN monocultures. Co-labeling of myelin basic proteins and neuronal cell microtubules reveals that SCs form myelin sheaths by wrapping around the axons of MNs. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 13 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 5 | 38% |
Unspecified | 1 | 8% |
Professor | 1 | 8% |
Student > Master | 1 | 8% |
Researcher | 1 | 8% |
Other | 2 | 15% |
Unknown | 2 | 15% |
Readers by discipline | Count | As % |
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Engineering | 2 | 15% |
Medicine and Dentistry | 2 | 15% |
Agricultural and Biological Sciences | 2 | 15% |
Veterinary Science and Veterinary Medicine | 1 | 8% |
Unspecified | 1 | 8% |
Other | 2 | 15% |
Unknown | 3 | 23% |