Chapter title |
Advances in Plant Gene Silencing Methods.
|
---|---|
Chapter number | 1 |
Book title |
Plant Gene Silencing
|
Published in |
Methods in molecular biology, February 2015
|
DOI | 10.1007/978-1-4939-2453-0_1 |
Pubmed ID | |
Book ISBNs |
978-1-4939-2452-3, 978-1-4939-2453-0
|
Authors |
Prachi Pandey, Muthappa Senthil-Kumar, Kirankumar S Mysore, Kirankumar S. Mysore, Pandey, Prachi, Senthil-Kumar, Muthappa, Mysore, Kirankumar S. |
Editors |
Kirankumar S. Mysore, Muthappa Senthil-Kumar |
Abstract |
Understanding molecular mechanisms of transcriptional and posttranscriptional gene silencing pathways in plants over the past decades has led to development of tools and methods for silencing a target gene in various plant species. In this review chapter, both the recent understanding of molecular basis of gene silencing pathways and advances in various widely used gene silencing methods are compiled. We also discuss the salient features of the different methods like RNA interference (RNAi) and virus-induced gene silencing (VIGS) and highlight their advantages and disadvantages. Gene silencing technology is constantly progressing as reflected by rapidly emerging new methods. A succinct discussion on the recently developed methods like microRNA-mediated virus-induced gene silencing (MIR-VIGS) and microRNA-induced gene silencing (MIGS) is also provided. One major bottleneck in gene silencing approaches has been the associated off-target silencing. The other hurdle has been the lack of a universal approach that can be applied to all plants. For example, we face hurdles like incompatibility of VIGS vectors with the host and inability to use MIGS for plant species which are not easily transformable. However, the overwhelming research in this direction reflects the scope for overcoming the short comings of gene silencing technology. |
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Geographical breakdown
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Unknown | 1 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 28 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 8 | 29% |
Student > Master | 4 | 14% |
Student > Bachelor | 4 | 14% |
Other | 2 | 7% |
Researcher | 2 | 7% |
Other | 2 | 7% |
Unknown | 6 | 21% |
Readers by discipline | Count | As % |
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Agricultural and Biological Sciences | 13 | 46% |
Biochemistry, Genetics and Molecular Biology | 6 | 21% |
Environmental Science | 1 | 4% |
Medicine and Dentistry | 1 | 4% |
Unknown | 7 | 25% |