Chapter title |
Induction of Stable Epigenetic Gene Silencing in Plants Using a Virus Vector.
|
---|---|
Chapter number | 9 |
Book title |
Plant Gene Silencing
|
Published in |
Methods in molecular biology, February 2015
|
DOI | 10.1007/978-1-4939-2453-0_9 |
Pubmed ID | |
Book ISBNs |
978-1-4939-2452-3, 978-1-4939-2453-0
|
Authors |
Akira Kanazawa, Megumi Kasai, Kanazawa, Akira, Kasai, Megumi |
Editors |
Kirankumar S. Mysore, Muthappa Senthil-Kumar |
Abstract |
Gene silencing through transcriptional repression can be induced by double-stranded RNA targeted to a gene promoter, a process known as RNA-mediated transcriptional gene silencing (TGS). This phenomenon is associated with epigenetic changes involving cytosine methylation of the promoter. Plant virus vectors have been used to induce RNA-mediated TGS. Here, we describe methods relevant to the induction of epigenetic changes and RNA-mediated TGS in plants using a virus vector, which include inoculation of recombinant virus, detection of short interfering RNAs, bisulfite sequencing analysis, and nuclear run-on transcription assay. |
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