Chapter title |
MR VIGS: MicroRNA-Based Virus-Induced Gene Silencing in Plants.
|
---|---|
Chapter number | 11 |
Book title |
Plant Gene Silencing
|
Published in |
Methods in molecular biology, February 2015
|
DOI | 10.1007/978-1-4939-2453-0_11 |
Pubmed ID | |
Book ISBNs |
978-1-4939-2452-3, 978-1-4939-2453-0
|
Authors |
Weiwei Chen, Qi Zhang, Junhua Kong, Feng Hu, Bin Li, Chaoqun Wu, Cheng Qin, Pengcheng Zhang, Nongnong Shi, Yiguo Hong, Chen, Weiwei, Zhang, Qi, Kong, Junhua, Hu, Feng, Li, Bin, Wu, Chaoqun, Qin, Cheng, Zhang, Pengcheng, Shi, Nongnong, Hong, Yiguo |
Editors |
Kirankumar S. Mysore, Muthappa Senthil-Kumar |
Abstract |
In plants, microRNA (miRNA)-based virus-induced gene silencing, dubbed MR VIGS, is a powerful technique to delineate the biological functions of genes. By targeting to a specific sequence, miRNAs can knock down expression of genes with fewer off-target effects. Here, using a modified Cabbage leaf curling virus (CaLCuV) and Tobacco rattle virus (TRV) as vectors, we describe two virus-based miRNA expression systems to perform MR VIGS for plant functional genomics assays. |
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