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Plant Gene Silencing

Overview of attention for book
Cover of 'Plant Gene Silencing'

Table of Contents

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    Book Overview
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    Chapter 1 Advances in Plant Gene Silencing Methods.
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    Chapter 2 Strategies for altering plant traits using virus-induced gene silencing technologies.
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    Chapter 3 Bioinformatics Tools for Achieving Better Gene Silencing in Plants.
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    Chapter 4 Profiling of Small RNAs Involved in Plant-Pathogen Interactions.
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    Chapter 5 RNAi-Mediated Resistance to Viruses in Genetically Engineered Plants.
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    Chapter 6 Simplifying Transgene Locus Structure Through Cre-lox Recombination.
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    Chapter 7 Transgene-Induced Gene Silencing in Plants.
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    Chapter 8 Gene silencing by DNA interference in fern gametophytes.
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    Chapter 9 Induction of Stable Epigenetic Gene Silencing in Plants Using a Virus Vector.
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    Chapter 10 A Method for Validating MicroRNAs in Plants by miR-RACE.
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    Chapter 11 MR VIGS: MicroRNA-Based Virus-Induced Gene Silencing in Plants.
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    Chapter 12 A High-Throughput RNA Interference (RNAi)-Based Approach Using Hairy Roots for the Study of Plant-Rhizobia Interactions.
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    Chapter 13 A Functional Genomics Method for Assaying Gene Function in Phytopathogenic Fungi Through Host-Induced Gene Silencing Mediated by Agroinfiltration.
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    Chapter 14 An Effective and Convenient Method for the Delivery of Apple Latent Spherical Virus (ALSV)-Based Vectors into Plant Cells by Agroinoculation.
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    Chapter 15 Virus-Induced Gene Silencing (VIGS) for Functional Genomics in Rice Using Rice tungro bacilliform virus (RTBV) as a Vector.
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    Chapter 16 Virus-induced gene silencing of fiber-related genes in cotton.
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    Chapter 17 Establishment of an Efficient Virus-Induced Gene Silencing (VIGS) Assay in Arabidopsis by Agrobacterium-Mediated Rubbing Infection.
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    Chapter 18 Virus-induced gene silencing as a scalable tool to study drought tolerance in plants.
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    Chapter 19 VIGS for Dissecting Mechanisms Involved in the Symbiotic Interaction of Microbes with Plants.
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    Chapter 20 Construction of a Cotton VIGS Library for Functional Genomics Study.
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    Chapter 21 Synthetic gene complementation to determine off-target silencing.
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    Chapter 22 Construction of Mismatched Inverted Repeat (IR) Silencing Vectors for Maximizing IR Stability and Effective Gene Silencing in Plants.
Attention for Chapter 7: Transgene-Induced Gene Silencing in Plants.
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Chapter title
Transgene-Induced Gene Silencing in Plants.
Chapter number 7
Book title
Plant Gene Silencing
Published in
Methods in molecular biology, February 2015
DOI 10.1007/978-1-4939-2453-0_7
Pubmed ID
25740359
Book ISBNs
978-1-4939-2452-3, 978-1-4939-2453-0
Authors

Yun Jin, Hui-Shan Guo, Jin, Yun, Guo, Hui-Shan

Editors

Kirankumar S. Mysore, Muthappa Senthil-Kumar

Abstract

RNAi is the most important reverse genetics tool to trigger transgenic gene silencing, which is now applied widely to investigate gene function and also practically applied to enhance resistance to biotic and abiotic stress. Recently, the most effective way to induce transgenic gene silencing is to introduce inverted repeat (IR) double-stranded RNA (dsRNA) or artificial microRNA (amiRNA) instead of a transgenic sense or antisense strand of genes. The stable transgenic plants can be acquired through Agrobacterium tumefaciens-mediated transformation of binary vectors containing an RNAi hairpin construct or amiRNA precursor backbone sequence. Here we primarily describe these two methods' vector construction, plant transformation, and transgenic line verification.

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X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
 Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 197 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
France 2 1%
United Kingdom 2 1%
Brazil 2 1%
United States 2 1%
Spain 1 <1%
Germany 1 <1%
Unknown 187 95%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 51 26%
Researcher 43 22%
Student > Master 22 11%
Student > Bachelor 14 7%
Student > Doctoral Student 11 6%
Other 36 18%
Unknown 20 10%
Readers by discipline Count As %
Agricultural and Biological Sciences 130 66%
Biochemistry, Genetics and Molecular Biology 34 17%
Earth and Planetary Sciences 2 1%
Medicine and Dentistry 2 1%
Computer Science 1 <1%
Other 5 3%
Unknown 23 12%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 06 March 2015.
All research outputs
#20,263,155
of 22,793,427 outputs
Outputs from Methods in molecular biology
#9,900
of 13,110 outputs
Outputs of similar age
#217,284
of 257,462 outputs
Outputs of similar age from Methods in molecular biology
#62
of 87 outputs
Altmetric has tracked 22,793,427 research outputs across all sources so far. This one is in the 1st percentile – i.e., 1% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,110 research outputs from this source. They receive a mean Attention Score of 3.3. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 257,462 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 87 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.

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