Chapter title |
An Effective and Convenient Method for the Delivery of Apple Latent Spherical Virus (ALSV)-Based Vectors into Plant Cells by Agroinoculation.
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Chapter number | 14 |
Book title |
Plant Gene Silencing
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Published in |
Methods in molecular biology, February 2015
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DOI | 10.1007/978-1-4939-2453-0_14 |
Pubmed ID | |
Book ISBNs |
978-1-4939-2452-3, 978-1-4939-2453-0
|
Authors |
Tatsuya Kon, Nubuyuki Yoshikawa, Kon, Tatsuya, Yoshikawa, Nubuyuki |
Editors |
Kirankumar S. Mysore, Muthappa Senthil-Kumar |
Abstract |
Virus infection leads to the synthesis of double-stranded RNA during virus replication, and then this infection produces small RNA molecules in the antiviral RNA silencing pathway. Here, we develop an Agrobacterium-mediated inoculation system for ALSV-based vectors. This system is more effective and convenient for inoculation of the ALSV vectors into plants compared to direct inoculation of ALSV-RNA2-based vectors in pUC plasmids reported previously. In addition, cointroduction of various plant viral RNA-silencing suppressors increased the efficiency of agroinoculation of the ALSV-based vector. An ALSV vector could be successfully used to silence an endogenous gene in plants. Therefore, the ALSV-based VIGS/agroinoculation system provides a valuable tool for functional genomics among a broad range of plant species. |
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Members of the public | 1 | 100% |
Mendeley readers
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Professor > Associate Professor | 1 | 20% |
Researcher | 1 | 20% |
Student > Doctoral Student | 1 | 20% |
Student > Master | 1 | 20% |
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Agricultural and Biological Sciences | 3 | 60% |
Biochemistry, Genetics and Molecular Biology | 1 | 20% |
Unknown | 1 | 20% |