Chapter title |
Separation of Recombinant Therapeutic Proteins Using Capillary Gel Electrophoresis and Capillary Isoelectric Focusing
|
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Chapter number | 11 |
Book title |
Capillary Electrophoresis of Proteins and Peptides
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-4014-1_11 |
Pubmed ID | |
Book ISBNs |
978-1-4939-4012-7, 978-1-4939-4014-1
|
Authors |
Caitlyn A. G. De Jong, Jessica Risley, Alexis K. Lee, Shuai Sherry Zhao, David D. Y. Chen |
Abstract |
Detailed step-by-step methods for protein separation techniques based on capillary electrophoresis (CE) are described in this chapter. Focus is placed on two techniques, capillary gel electrophoresis (CGE) and capillary isoelectric focusing (cIEF). CGE is essentially gel electrophoresis, performed in a capillary, where a hydrogel is used as a sieving matrix to separate proteins or peptides based on size. cIEF separates proteins or peptides based on their isoelectric point (pI), the pH at which the protein or peptide bears no charges. Detailed protocols and steps (including capillary preparation, sample preparation, CE separation conditions, and detection) for both CGE and cIEF presented so that readers can follow the described methods in their own labs. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 13 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Bachelor | 4 | 31% |
Student > Master | 2 | 15% |
Student > Doctoral Student | 2 | 15% |
Unspecified | 1 | 8% |
Student > Ph. D. Student | 1 | 8% |
Other | 1 | 8% |
Unknown | 2 | 15% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 3 | 23% |
Chemistry | 2 | 15% |
Pharmacology, Toxicology and Pharmaceutical Science | 2 | 15% |
Chemical Engineering | 1 | 8% |
Unspecified | 1 | 8% |
Other | 2 | 15% |
Unknown | 2 | 15% |