| Chapter title |
Computational Prediction of New Intein Split Sites
|
|---|---|
| Chapter number | 17 |
| Book title |
Split Inteins
|
| Published in |
Methods in molecular biology, January 2017
|
| DOI | 10.1007/978-1-4939-6451-2_17 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6449-9, 978-1-4939-6451-2
|
| Authors |
Yi-Zong Lee, Wei-Cheng Lo, Shih-Che Sue, Lee, Yi-Zong, Lo, Wei-Cheng, Sue, Shih-Che |
| Abstract |
Split inteins have emerged as a powerful tool in protein engineering. We describe a reliable in silico method to predict viable split sites for the design of new split inteins. A computational circular permutation (CP) prediction method facilitates the search for internal permissive sites to create artificial circular permutants. In this procedure, the original amino- and carboxyl-termini are connected and new termini are created. The identified new terminal sites are promising candidates for the generation of new split sites with the backbone opening being tolerated by the structural scaffold. Here we show how to integrate the online usage of the CP predictor, CPred, in the search of new split intein sites. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Germany | 1 | 17% |
| Unknown | 5 | 83% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 1 | 17% |
| Student > Bachelor | 1 | 17% |
| Researcher | 1 | 17% |
| Student > Master | 1 | 17% |
| Unknown | 2 | 33% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 1 | 17% |
| Computer Science | 1 | 17% |
| Agricultural and Biological Sciences | 1 | 17% |
| Medicine and Dentistry | 1 | 17% |
| Unknown | 2 | 33% |