| Chapter title |
Photocontrol of the Src Kinase in Mammalian Cells with a Photocaged Intein
|
|---|---|
| Chapter number | 14 |
| Book title |
Split Inteins
|
| Published in |
Methods in molecular biology, January 2017
|
| DOI | 10.1007/978-1-4939-6451-2_14 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6449-9, 978-1-4939-6451-2
|
| Authors |
Wei Ren, Hui-Wang Ai, Ren, Wei, Ai, Hui-Wang |
| Abstract |
Recently developed methods that can photochemically control protein activities and functions in live cells have opened up new opportunities for studying signaling networks at the cellular and subcellular levels. Our laboratory has reported a genetically encoded photoactivatable intein, which allows the direct photocontrol of primary sequences of proteins, and consequently, their activities and functions in live mammalian cells. Herein, we provide details on experimental design and the utilization of this photocaged intein to photoactivate the Src tyrosine kinase in human embryonic kidney (HEK) 293T cells. The described procedures may be adopted to photocontrol other proteins in other types of mammalian cells. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 7 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 2 | 29% |
| Student > Bachelor | 1 | 14% |
| Student > Master | 1 | 14% |
| Unknown | 3 | 43% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 1 | 14% |
| Materials Science | 1 | 14% |
| Unknown | 5 | 71% |