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Mendeley readers
| Chapter title |
Purification of Microbially Expressed Recombinant Proteins via a Dual ELP Split Intein System
|
|---|---|
| Chapter number | 2 |
| Book title |
Split Inteins
|
| Published in |
Methods in molecular biology, January 2017
|
| DOI | 10.1007/978-1-4939-6451-2_2 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6449-9, 978-1-4939-6451-2
|
| Authors |
Changhua Shi, Tzu-Chiang Han, David W. Wood, Shi, Changhua, Han, Tzu-Chiang, Wood, David W. |
| Abstract |
Fusions of elastin-like peptide (ELP) purification tags and self-cleaving inteins provide a powerful platform for purifying tagless recombinant proteins without the need for conventional packed-bed columns. A drawback to this method has been premature cleaving of the ELP tag during expression, before the purification procedure can take place. Here we demonstrate a split-intein method, where the self-cleaving intein is divided into two inactive segments during expression and purification. Spontaneous assembly of the purified intein segments then restores self-cleaving activity to deliver the tagless target protein. |
Mendeley readers
The data shown below were compiled from readership statistics for 5 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 5 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 2 | 40% |
| Student > Bachelor | 1 | 20% |
| Student > Doctoral Student | 1 | 20% |
| Unknown | 1 | 20% |
| Readers by discipline | Count | As % |
|---|---|---|
| Chemical Engineering | 1 | 20% |
| Biochemistry, Genetics and Molecular Biology | 1 | 20% |
| Agricultural and Biological Sciences | 1 | 20% |
| Unknown | 2 | 40% |