Chapter title |
Laser Capture Microdissection as a Tool to Study the Mucosal Immune Response in Celiac Disease
|
---|---|
Chapter number | 7 |
Book title |
Laser Capture Microdissection
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-7558-7_7 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7557-0, 978-1-4939-7558-7
|
Authors |
Giuseppe Iacomino, Vera Rotondi Aufiero, Pasquale Marena, Antonella Venezia, Riccardo Troncone, Salvatore Auricchio, Giuseppe Mazzarella, Iacomino, Giuseppe, Aufiero, Vera Rotondi, Marena, Pasquale, Venezia, Antonella, Troncone, Riccardo, Auricchio, Salvatore, Mazzarella, Giuseppe |
Abstract |
Laser capture microdissection (LCM) is a powerful tool for selection and isolation of single cells or compartments from complex primary tissues to perform molecular analyses. Celiac disease is a genetic autoimmune disorder where the ingestion of gluten leads to damage in the small intestine. Increased intraepithelial lymphocytes and the presence of the lamina propria inflammatory infiltrate of the duodenal mucosa is a common part of the disease. These cells promote inflammatory processes through the release of cytokines. Here, we describe the use of LCM and real-time quantitative PCR (RT-qPCR) to analyze cytokine profile information in distinct duodenal mucosa tissue compartments of celiac patients. |
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Unknown | 8 | 100% |
Demographic breakdown
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Researcher | 3 | 38% |
Professor | 1 | 13% |
Student > Doctoral Student | 1 | 13% |
Unknown | 3 | 38% |
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Unknown | 3 | 38% |