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Laser Capture Microdissection

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Cover of 'Laser Capture Microdissection'

Table of Contents

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    Book Overview
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    Chapter 1 Laser Capture Microdissection: Insights into Methods and Applications
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    Chapter 2 Laser Microdissection-Based Microproteomics of Formalin-Fixed and Paraffin-Embedded (FFPE) Tissues
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    Chapter 3 Laser Microdissection Workflow for Isolating Nucleic Acids from Fixed and Frozen Tissue Samples
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    Chapter 4 Protocol for the Analysis of Laser Capture Microdissected Fresh-Frozen Tissue Homogenates by Silver-Stained 1D SDS-PAGE
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    Chapter 5 Next-Generation Sequencing Analysis of Laser-Microdissected Formalin-Fixed and Paraffin-Embedded (FFPE) Tissue Specimens
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    Chapter 6 Adaptation of Laser Microdissection Technique to Nanostring RNA Analysis in the Study of a Spontaneous Metastatic Mammary Carcinoma Mouse Model
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    Chapter 7 Laser Capture Microdissection as a Tool to Study the Mucosal Immune Response in Celiac Disease
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    Chapter 8 Laser Capture Microdissection and Isolation of High-Quality RNA from Frozen Endometrial Tissue
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    Chapter 9 Laser Microdissection for Human Papillomavirus (HPV) Genotyping Attribution and Methylation Pattern Analyses of Squamous Intraepithelial Lesions
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    Chapter 10 Laser Capture Microdissection and Transcriptional Analysis of Sub-Populations of the Osteoblast Lineage from Undecalcified Bone
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    Chapter 11 Cell Type-Specific Laser Capture Microdissection for Gene Expression Profiling in the Human Brain
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    Chapter 12 The Isolation of Pure Populations of Neurons by Laser Capture Microdissection: Methods and Application in Neuroscience
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    Chapter 13 Laser Capture Microdissection in Traumatic Brain Injury Research: Obtaining Hippocampal Subregions and Pools of Injured Neurons for Genomic Analyses
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    Chapter 14 Isolation of Distinct Types of Neurons from Fresh Brain Tissue Using Laser Microdissection in Combination with High-Performance Liquid Chromatography—Mass Spectrometry
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    Chapter 15 Immuno-Guided Laser-Capture Microdissection of Glial Cells for mRNA Analysis
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    Chapter 16 Immuno-Laser-Capture Microdissection for the Isolation of Enriched Glial Populations from Frozen Post-Mortem Human Brain
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    Chapter 17 Laser-Capture Microdissection for the Analysis of Rat and Human Spinal Cord Ependyma by qPCR
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    Chapter 18 Isolation of Amyloid Plaques and Neurofibrillary Tangles from Archived Alzheimer’s Disease Tissue Using Laser-Capture Microdissection for Downstream Proteomics
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    Chapter 19 Cell-Specific RNA Quantification in Human SN DA Neurons from Heterogeneous Post-mortem Midbrain Samples by UV-Laser Microdissection and RT-qPCR
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    Chapter 20 Laser-Capture Microdissection for Layer-Specific Analysis of Enteric Ganglia
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    Chapter 21 A Laser Microdissection–Liquid Chromatography–Tandem Mass Spectrometry Workflow for Post-mortem Analysis of Brain Tissue
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    Chapter 22 Laser-Capture Microdissection and RNA Extraction from Perfusion-Fixed Cartilage and Bone Tissue from Mice Implanted with Human iPSC-Derived MSCs in a Calvarial Defect Model
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    Chapter 23 Laser Capture Microdissection-Based RNA-Seq of Barley Grain Tissues
Attention for Chapter 5: Next-Generation Sequencing Analysis of Laser-Microdissected Formalin-Fixed and Paraffin-Embedded (FFPE) Tissue Specimens
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Chapter title
Next-Generation Sequencing Analysis of Laser-Microdissected Formalin-Fixed and Paraffin-Embedded (FFPE) Tissue Specimens
Chapter number 5
Book title
Laser Capture Microdissection
Published in
Methods in molecular biology, January 2018
DOI 10.1007/978-1-4939-7558-7_5
Pubmed ID
29344856
Book ISBNs
978-1-4939-7557-0, 978-1-4939-7558-7
Authors

Lavinia Mägel, Stephan Bartels, Ulrich Lehmann, Mägel, Lavinia, Bartels, Stephan, Lehmann, Ulrich

Abstract

In recent years, next-generation sequencing (NGS) became widely used in molecular pathology. Comprehensive mutational profiling improved diagnosis and prognosis, as well as the identification of therapeutically relevant genetic alterations. However, the vast majority of studies analyzing tissue samples use DNA extracted from bulk tissue or only manually microdissected specimens. Laser-assisted microdissection offers the possibility of isolating morphologically defined small tissue compartments (like individual glands) or even of single cells for further molecular analysis. Even formalin-fixed paraffin-embedded (FFPE) tissue specimens can be used for laser-assisted microdissection. Combining these two innovative powerful methodological approaches provides invaluable insights into the genetic profile of any cell type and tissue compartment of interest, contributing to a better understanding of fundamental biological processes and disease-specific mechanisms.In this chapter, a detailed protocol is provided for microdissection of human mammary adenomyoepithelioma tissue specimens and subsequent targeted resequencing of a panel of cancer-related genes using IonTorrent/PGM technology.

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X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
 Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 9 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 9 100%

Demographic breakdown

Readers by professional status Count As %
Student > Master 2 22%
Other 2 22%
Professor 1 11%
Student > Bachelor 1 11%
Unknown 3 33%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 3 33%
Medicine and Dentistry 2 22%
Pharmacology, Toxicology and Pharmaceutical Science 1 11%
Unknown 3 33%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 17 January 2019.
All research outputs
#20,483,282
of 23,045,021 outputs
Outputs from Methods in molecular biology
#9,975
of 13,194 outputs
Outputs of similar age
#378,267
of 442,426 outputs
Outputs of similar age from Methods in molecular biology
#1,194
of 1,499 outputs
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So far Altmetric has tracked 13,194 research outputs from this source. They receive a mean Attention Score of 3.4. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
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