Chapter title |
A Laser Microdissection–Liquid Chromatography–Tandem Mass Spectrometry Workflow for Post-mortem Analysis of Brain Tissue
|
---|---|
Chapter number | 21 |
Book title |
Laser Capture Microdissection
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-7558-7_21 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7557-0, 978-1-4939-7558-7
|
Authors |
David C. Hondius, Jeroen J. M. Hoozemans, Annemieke J. M. Rozemuller, Ka Wan Li, August B. Smit, Hondius, David C., Hoozemans, Jeroen J. M., Rozemuller, Annemieke J. M., Li, Ka Wan, Smit, August B. |
Abstract |
Improved speed and sensitivity of mass spectrometry allow the simultaneous quantification of high numbers of proteins from increasingly smaller quantities of tissue sample. Quantitative data of the proteome is highly valuable for providing unbiased information on, for example, protein expression changes related to disease or identifying related biomarkers. In brain diseases the affected area can be small and pathogenic events can be related to a specific cell type in an otherwise heterogeneous tissue type. An emerging approach dedicated to analyzing this type of samples is laser micro-dissection (LMD) combined with LC-MS/MS into a single workflow. In this chapter, we describe different options for isolating tissue suitable for LC-MS/MS analysis. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 19 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 4 | 21% |
Other | 3 | 16% |
Researcher | 3 | 16% |
Student > Bachelor | 2 | 11% |
Professor > Associate Professor | 2 | 11% |
Other | 2 | 11% |
Unknown | 3 | 16% |
Readers by discipline | Count | As % |
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Neuroscience | 6 | 32% |
Biochemistry, Genetics and Molecular Biology | 4 | 21% |
Agricultural and Biological Sciences | 1 | 5% |
Business, Management and Accounting | 1 | 5% |
Chemistry | 1 | 5% |
Other | 1 | 5% |
Unknown | 5 | 26% |