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Chapter title |
Immuno-Laser-Capture Microdissection for the Isolation of Enriched Glial Populations from Frozen Post-Mortem Human Brain
|
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Chapter number | 16 |
Book title |
Laser Capture Microdissection
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Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-7558-7_16 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7557-0, 978-1-4939-7558-7
|
Authors |
Julie E. Simpson, Stephen B. Wharton, Paul R. Heath, Simpson, Julie E., Wharton, Stephen B., Heath, Paul R. |
Abstract |
Laser-capture microdissection (LCM) has revolutionized the isolation of defined regions and specific cell populations from human tissue. This approach used in combination with immunohistochemistry (immuno-LCM) has become a valuable method for isolating individual cell-types from a complex heterogeneous population. Here, we describe the detailed methodology required for the isolation of enriched populations of GFAP+ astrocytes, OSP+ oligodendrocytes, and CD68+ microglia from frozen post-mortem human central nervous system tissue using immuno-LCM. |
Mendeley readers
The data shown below were compiled from readership statistics for 9 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 9 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 3 | 33% |
Researcher | 3 | 33% |
Student > Bachelor | 1 | 11% |
Student > Master | 1 | 11% |
Lecturer > Senior Lecturer | 1 | 11% |
Other | 0 | 0% |
Readers by discipline | Count | As % |
---|---|---|
Neuroscience | 4 | 44% |
Biochemistry, Genetics and Molecular Biology | 3 | 33% |
Medicine and Dentistry | 1 | 11% |
Design | 1 | 11% |