Chapter title |
Encapsulation of Date Palm Somatic Embryos: Synthetic Seeds
|
---|---|
Chapter number | 7 |
Book title |
Date Palm Biotechnology Protocols Volume II
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-7159-6_7 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7158-9, 978-1-4939-7159-6
|
Authors |
Shawky A. Bekheet |
Abstract |
Synthetic seed or encapsulated somatic embryos may be used for propagation, storage, and exchange of plant germplasm and have many diverse applications in date palm cultivation. They have advantages over conventional use of offshoot material for germplasm propagation, maintenance, exchange, and transportation. This chapter describes a protocol for date palm synthetic seed production by encapsulation of somatic embryos with sodium alginate. Among three concentrations used, 3% sodium alginate followed by dropping into 2.5% calcium chloride (CaCl2) solution shows the best concentration of gel matrix for both maintenance and recovery. In addition, storage of the encapsulated date palm somatic embryos at 5 °C improves the survival and conversion into plantlets; otherwise, 20 g/L sucrose in the culture medium enhances conversion of the recovered somatic embryos to plantlets. This protocol is promising for in vitro conservation and international exchange of date palm germplasm. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 8 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Unspecified | 1 | 13% |
Professor | 1 | 13% |
Student > Doctoral Student | 1 | 13% |
Unknown | 5 | 63% |
Readers by discipline | Count | As % |
---|---|---|
Unspecified | 1 | 13% |
Biochemistry, Genetics and Molecular Biology | 1 | 13% |
Unknown | 6 | 75% |