Chapter title |
Storage and Viability Assessment of Date Palm Pollen
|
---|---|
Chapter number | 1 |
Book title |
Date Palm Biotechnology Protocols Volume II
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-7159-6_1 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7158-9, 978-1-4939-7159-6
|
Authors |
Maryam, Muhammad J. Jaskani, Summar A. Naqvi |
Abstract |
Pollen storage and viability are very important for pollination, breeding, biodiversity, biotechnology, conservation, and other biological and non-biological studies of the date palm. Optimizing procedures and duration of storage are important for effective and long-term date palm pollen storage and viability. Here we describe pollen storage methods, such as room temperature (25-30 °C), refrigeration (4 °C), storage at 4 °C in desiccators, deep freezer (-20 °C), and cryopreservation (-196 °C). Based on pollen viability by staining and in vitro germination methods, cryopreservation is the best method for long-term storage without any significant effect on pollen viability (75-84%); however, the percentage of pollen viability depends on the storage period. |
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