Chapter title |
Determining Phylogenetic Relationships Among Date Palm Cultivars Using Random Amplified Polymorphic DNA (RAPD) and Inter-Simple Sequence Repeat (ISSR) Markers
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Chapter number | 14 |
Book title |
Date Palm Biotechnology Protocols Volume II
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Published in |
Methods in molecular biology, January 2017
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DOI | 10.1007/978-1-4939-7159-6_14 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7158-9, 978-1-4939-7159-6
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Authors |
Nadia Haider |
Abstract |
Investigation of genetic variation and phylogenetic relationships among date palm (Phoenix dactylifera L.) cultivars is useful for their conservation and genetic improvement. Various molecular markers such as restriction fragment length polymorphisms (RFLPs), simple sequence repeat (SSR), representational difference analysis (RDA), and amplified fragment length polymorphism (AFLP) have been developed to molecularly characterize date palm cultivars. PCR-based markers random amplified polymorphic DNA (RAPD) and inter-simple sequence repeat (ISSR) are powerful tools to determine the relatedness of date palm cultivars that are difficult to distinguish morphologically. In this chapter, the principles, materials, and methods of RAPD and ISSR techniques are presented. Analysis of data generated from these two techniques and the use of these data to reveal phylogenetic relationships among date palm cultivars are also discussed. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 7 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Researcher | 2 | 29% |
Unspecified | 1 | 14% |
Student > Ph. D. Student | 1 | 14% |
Student > Doctoral Student | 1 | 14% |
Student > Master | 1 | 14% |
Other | 1 | 14% |
Readers by discipline | Count | As % |
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Unspecified | 1 | 14% |
Unknown | 1 | 14% |