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RNA Nanotechnology and Therapeutics

Overview of attention for book
Cover of 'RNA Nanotechnology and Therapeutics'

Table of Contents

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    Book Overview
  2. Altmetric Badge
    Chapter 1 Overview of methods in RNA nanotechnology: synthesis, purification, and characterization of RNA nanoparticles.
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    Chapter 2 Multiple Approaches for the Investigation of Bacterial Small Regulatory RNAs Self-assembly.
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    Chapter 3 Measuring the Elasticity of Ribonucleotide(s)-Containing DNA Molecules Using AFM.
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    Chapter 4 Silver nanoclusters for RNA nanotechnology: steps towards visualization and tracking of RNA nanoparticle assemblies.
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    Chapter 5 Large scale purification of RNA nanoparticles by preparative ultracentrifugation.
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    Chapter 6 HPLC Purification of RNA Aptamers up to 59 Nucleotides with Single-Nucleotide Resolution
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    Chapter 7 Using RNA nanoparticles with thermostable motifs and fluorogenic modules for real-time detection of RNA folding and turnover in prokaryotic and eukaryotic cells.
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    Chapter 8 Fluorescence Labeling of Short RNA by Oxidation at the 3′-End
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    Chapter 9 Methods and assays for specific targeting and delivery of RNA nanoparticles to cancer metastases.
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    Chapter 10 Functional assays for specific targeting and delivery of RNA nanoparticles to brain tumor.
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    Chapter 11 Aptamer-Mediated Nanoparticle Interactions: From Oligonucleotide–Protein Complexes to SELEX Screens
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    Chapter 12 Methods for Assembling B-Cell Lymphoma Specific and Internalizing Aptamer-siRNA Nanoparticles Via the Sticky Bridge.
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    Chapter 13 A high-throughput screening assay for the functional delivery of splice-switching oligonucleotides in human melanoma cells.
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    Chapter 14 Design, Assembly, and Evaluation of RNA-Protein Nanostructures.
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    Chapter 15 Mapping RNA Interactions to Proteins in Virions Using CLIP-Seq
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    Chapter 16 Mapping Protein–RNA Interactions by RCAP, RNA-Cross-Linking and Peptide Fingerprinting
Attention for Chapter 5: Large scale purification of RNA nanoparticles by preparative ultracentrifugation.
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About this Attention Score

  • Good Attention Score compared to outputs of the same age (77th percentile)
  • High Attention Score compared to outputs of the same age and source (86th percentile)

Mentioned by

blogs
1 blog

Citations

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5 Dimensions

Readers on

mendeley
16 Mendeley
Summary Blogs Dimensions citations
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Chapter title
Large scale purification of RNA nanoparticles by preparative ultracentrifugation.
Chapter number 5
Book title
RNA Nanotechnology and Therapeutics
Published in
Methods in molecular biology, January 2015
DOI 10.1007/978-1-4939-2562-9_5
Pubmed ID
25895996
Book ISBNs
978-1-4939-2561-2, 978-1-4939-2562-9
Authors

Jasinski, Daniel L, Schwartz, Chad T, Haque, Farzin, Guo, Peixuan, Daniel L. Jasinski, Chad T. Schwartz, Farzin Haque, Peixuan Guo, Jasinski, Daniel L., Schwartz, Chad T.

Abstract

Purification of large quantities of supramolecular RNA complexes is of paramount importance due to the large quantities of RNA needed and the purity requirements for in vitro and in vivo assays. Purification is generally carried out by liquid chromatography (HPLC), polyacrylamide gel electrophoresis (PAGE), or agarose gel electrophoresis (AGE). Here, we describe an efficient method for the large-scale purification of RNA prepared by in vitro transcription using T7 RNA polymerase by cesium chloride (CsCl) equilibrium density gradient ultracentrifugation and the large-scale purification of RNA nanoparticles by sucrose gradient rate-zonal ultracentrifugation or cushioned sucrose gradient rate-zonal ultracentrifugation.

View on publisher site
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 16 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 16 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 5 31%
Student > Bachelor 4 25%
Researcher 2 13%
Student > Master 1 6%
Unknown 4 25%
Readers by discipline Count As %
Agricultural and Biological Sciences 3 19%
Chemical Engineering 2 13%
Medicine and Dentistry 2 13%
Chemistry 2 13%
Biochemistry, Genetics and Molecular Biology 1 6%
Other 4 25%
Unknown 2 13%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 6. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 12 February 2020.
All research outputs
#5,754,695
of 22,854,458 outputs
Outputs from Methods in molecular biology
#1,623
of 13,128 outputs
Outputs of similar age
#77,215
of 353,257 outputs
Outputs of similar age from Methods in molecular biology
#126
of 997 outputs
Altmetric has tracked 22,854,458 research outputs across all sources so far. This one has received more attention than most of these and is in the 74th percentile.
So far Altmetric has tracked 13,128 research outputs from this source. They receive a mean Attention Score of 3.4. This one has done well, scoring higher than 87% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 353,257 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 77% of its contemporaries.
We're also able to compare this research output to 997 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 86% of its contemporaries.

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