Chapter title |
Methods for Assembling B-Cell Lymphoma Specific and Internalizing Aptamer-siRNA Nanoparticles Via the Sticky Bridge.
|
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Chapter number | 12 |
Book title |
RNA Nanotechnology and Therapeutics
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Published in |
Methods in molecular biology, January 2015
|
DOI | 10.1007/978-1-4939-2562-9_12 |
Pubmed ID | |
Book ISBNs |
978-1-4939-2561-2, 978-1-4939-2562-9
|
Authors |
Zhou, Jiehua, Rossi, John J, Shum, Ka To, Jiehua Zhou, John J. Rossi, Ka To Shum, Rossi, John J. |
Abstract |
Structured functional RNA entities, including aptamers and siRNAs, have amazing versatility in structure and function. These molecules can serve as powerful, attractive building blocks for the bottom-up assembly of complex nanostructures. Here, we describe novel cell-type specific and internalizing B-cell activating factor receptor (BAFF-R) aptamer-siRNA delivery systems for B-cell lymphoma therapy, in which both the aptamer and the Dicer substrate siRNA (DsiRNA) portions are conjugated through a "sticky bridge." The BAFF-R is overexpressed on the surface of B-cell malignancies, allowing binding and internalization of the aptamer-stick-siRNA nanoparticles. STAT3 siRNAs are encapsulated within the nanoparticles delivered by the BAFF-R aptamers and are localized to the cytoplasm, resulting in robust gene silencing of STAT3 mRNAs in a variety of B-cell lines. Moreover, these nanoparticles do not induce cell proliferation and apoptosis. Collectively, aptamer-mediated delivery strategies provide a toolset to become a more widely used therapeutic modality for the treatment of diseases. |
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