Chapter title |
Aptamer-Mediated Nanoparticle Interactions: From Oligonucleotide–Protein Complexes to SELEX Screens
|
---|---|
Chapter number | 11 |
Book title |
RNA Nanotechnology and Therapeutics
|
Published in |
Methods in molecular biology, January 2015
|
DOI | 10.1007/978-1-4939-2562-9_11 |
Pubmed ID | |
Book ISBNs |
978-1-4939-2561-2, 978-1-4939-2562-9
|
Authors |
Laetitia Evadé, Eric Dausse, Said Taouji, Emilie Daguerre, Eric Chevet, Jean-Jacques Toulmé, Evadé, Laetitia, Dausse, Eric, Taouji, Said, Daguerre, Emilie, Chevet, Eric, Toulmé, Jean-Jacques |
Abstract |
Aptamers are oligonucleotides displaying specific binding properties for a predetermined target. They can be easily immobilized on various surfaces such as nanoparticles. Functionalized particles can then be used to various aims. We took advantage of the AlphaScreen(®) technology for monitoring aptamer-mediated interactions. A particle bearing an aptamer contains a photosensitizer whereas another type of particle contains a chemiluminescer. Irradiation causes the formation of singlet oxygen species in the photosensitizer-containing bead that in turn activates the chemiluminescer. Luminescence emission can be observed if the two types of beads are in close proximity (<200 nm). This is achieved when the cognate ligand of the aptamer is grafted onto the chemiluminescer-containing bead. Using this technology we have screened oligonucleotide libraries and monitored aptamer-protein interactions. This constitutes the basis for aptamer-based analytical assays. |
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