Chapter title |
Detailed Protocols for the Isolation, Culture, Enrichment and Immunostaining of Primary Human Schwann Cells
|
---|---|
Chapter number | 5 |
Book title |
Schwann Cells
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-7649-2_5 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7648-5, 978-1-4939-7649-2
|
Authors |
Tamara Weiss, Sabine Taschner-Mandl, Peter F. Ambros, Inge M. Ambros, Weiss, Tamara, Taschner-Mandl, Sabine, Ambros, Peter F., Ambros, Inge M. |
Abstract |
This chapter emphasizes detailed protocols for the effective establishment of highly enriched human Schwann cell cultures and their characterization via immunostaining. The Schwann cells are isolated from immediately dissociated fascicle tissue and expanded prior to purification. Two purification methods are described that use either fluorescence-activated cell sorting for the Schwann cell marker TNR16 (p75NTR) or a less-manipulative two-step enrichment exploiting the differential adhesion properties of Schwann cells and fibroblasts, which is especially useful for low Schwann cell numbers. In addition, a method to determine Schwann cell purity via stained cytospin slides is introduced. Together with an immunofluorescence staining procedure for the combined analysis of extra- and intracellular markers, this chapter provides a solid basis to study human primary Schwann cells. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 26 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Bachelor | 4 | 15% |
Researcher | 4 | 15% |
Student > Ph. D. Student | 4 | 15% |
Student > Doctoral Student | 3 | 12% |
Student > Postgraduate | 2 | 8% |
Other | 3 | 12% |
Unknown | 6 | 23% |
Readers by discipline | Count | As % |
---|---|---|
Medicine and Dentistry | 5 | 19% |
Neuroscience | 4 | 15% |
Biochemistry, Genetics and Molecular Biology | 2 | 8% |
Agricultural and Biological Sciences | 1 | 4% |
Social Sciences | 1 | 4% |
Other | 3 | 12% |
Unknown | 10 | 38% |