Chapter title |
3D Cancer Migration Assay with Schwann Cells
|
---|---|
Chapter number | 20 |
Book title |
Schwann Cells
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-7649-2_20 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7648-5, 978-1-4939-7649-2
|
Authors |
Laura Fangmann, Steffen Teller, Pavel Stupakov, Helmut Friess, Güralp O. Ceyhan, Ihsan Ekin Demir, Fangmann, Laura, Teller, Steffen, Stupakov, Pavel, Friess, Helmut, Ceyhan, Güralp O., Demir, Ihsan Ekin |
Abstract |
In pancreatic cancer, neural invasion is one of the most common paths of cancer dissemination. Classically, cancer cells actively invade nerves and cause local recurrence and pain. Three-dimensional (3D) neural migration assay has become a standard tool for scientists to study neural invasion by confronting the involved cell types. This protocol introduces Schwann cells, i.e., the most prevalent cell type in peripheral nerves, in a novel heterotypic, glia-cancer-neuron, 3D migration assay for assessing their relevance in the early pathogenesis of neural invasion. Particularly, this assay allows the monitoring of the early Schwann cell migratory activity. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 12 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 4 | 33% |
Student > Ph. D. Student | 3 | 25% |
Student > Doctoral Student | 1 | 8% |
Student > Master | 1 | 8% |
Unknown | 3 | 25% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 5 | 42% |
Neuroscience | 2 | 17% |
Chemical Engineering | 1 | 8% |
Medicine and Dentistry | 1 | 8% |
Unknown | 3 | 25% |