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Chapter title |
Teased Fiber Preparation of Myelinated Nerve Fibers from Peripheral Nerves for Vital Dye Staining and Immunofluorescence Analysis
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Chapter number | 21 |
Book title |
Schwann Cells
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Published in |
Methods in molecular biology, January 2018
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DOI | 10.1007/978-1-4939-7649-2_21 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7648-5, 978-1-4939-7649-2
|
Authors |
Alejandra Catenaccio, Felipe A. Court, Catenaccio, Alejandra, Court, Felipe A. |
Abstract |
Glial cells regulate a wide variety of neuronal functions during physiological and pathological conditions. Therefore, the study of glial cells and their association with axons is of paramount importance in order to understand the physiology of the nervous system. This chapter describes a detailed protocol to prepare and stain teased nerve fibers from peripheral nerves using fluorescent indirect immunolabeling and staining with vital dyes. For immunofluorescence analysis, we describe techniques to study the axonal compartment and the expression of cytoplasmic and plasma membrane proteins in Schwann cells. |
Mendeley readers
The data shown below were compiled from readership statistics for 11 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 11 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 3 | 27% |
Student > Bachelor | 1 | 9% |
Student > Ph. D. Student | 1 | 9% |
Other | 1 | 9% |
Unknown | 5 | 45% |
Readers by discipline | Count | As % |
---|---|---|
Neuroscience | 3 | 27% |
Agricultural and Biological Sciences | 2 | 18% |
Chemical Engineering | 1 | 9% |
Unknown | 5 | 45% |