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Schwann Cells

Overview of attention for book
Cover of 'Schwann Cells'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 Isolation of Schwann Cell Precursors from Rodents
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    Chapter 2 Preparation of Neonatal Rat Schwann Cells and Embryonic Dorsal Root Ganglia Neurons for In Vitro Myelination Studies
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    Chapter 3 Isolation and Expansion of Schwann Cells from Transgenic Mouse Models
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    Chapter 4 Isolation, Culture, and Cryopreservation of Adult Rodent Schwann Cells Derived from Immediately Dissociated Teased Fibers
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    Chapter 5 Detailed Protocols for the Isolation, Culture, Enrichment and Immunostaining of Primary Human Schwann Cells
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    Chapter 6 Magnetic-Activated Cell Sorting for the Fast and Efficient Separation of Human and Rodent Schwann Cells from Mixed Cell Populations
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    Chapter 7 Culture and Expansion of Rodent and Porcine Schwann Cells for Preclinical Animal Studies
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    Chapter 8 Chemical Conversion of Human Fibroblasts into Functional Schwann Cells
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    Chapter 9 Derivation of Fate-Committed Schwann Cells from Bone Marrow Stromal Cells of Adult Rats
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    Chapter 10 Human Induced Pluripotent Stem Cell-Derived Sensory Neurons for Fate Commitment of Bone Marrow Stromal Cell-Derived Schwann Cells
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    Chapter 11 Generation and Use of Merlin-Deficient Human Schwann Cells for a High-Throughput Chemical Genomics Screening Assay
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    Chapter 12 Lentiviral Transduction of Rat Schwann Cells and Dorsal Root Ganglia Neurons for In Vitro Myelination Studies
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    Chapter 13 Preservation, Sectioning, and Staining of Schwann Cell Cultures for Transmission Electron Microscopy Analysis
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    Chapter 14 Scalable Differentiation and Dedifferentiation Assays Using Neuron-Free Schwann Cell Cultures
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    Chapter 15 The Pseudopod System for Axon-Glia Interactions: Stimulation and Isolation of Schwann Cell Protrusions that Form in Response to Axonal Membranes
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    Chapter 16 In Vitro Analysis of the Role of Schwann Cells on Axonal Degeneration and Regeneration Using Sensory Neurons from Dorsal Root Ganglia
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    Chapter 17 A Culture Model to Study Neuron-Schwann Cell-Astrocyte Interactions
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    Chapter 18 Preparation of Matrices of Variable Stiffness for the Study of Mechanotransduction in Schwann Cell Development
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    Chapter 19 Purification of Exosomes from Primary Schwann Cells, RNA Extraction, and Next-Generation Sequencing of Exosomal RNAs
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    Chapter 20 3D Cancer Migration Assay with Schwann Cells
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    Chapter 21 Teased Fiber Preparation of Myelinated Nerve Fibers from Peripheral Nerves for Vital Dye Staining and Immunofluorescence Analysis
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    Chapter 22 Whole Mount Immunostaining on Mouse Sciatic Nerves to Visualize Events of Peripheral Nerve Regeneration
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    Chapter 23 The Use of Low Vacuum Scanning Electron Microscopy (LVSEM) to Analyze Peripheral Nerve Samples
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    Chapter 24 Analysis of Myelinating Schwann Cells in Human Skin Biopsies
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    Chapter 25 Whole Mount In Situ Hybridization and Immunohistochemistry for Zebrafish Larvae
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    Chapter 26 Transmission Electron Microscopy for Zebrafish Larvae and Adult Lateral Line Nerve
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    Chapter 27 Live Imaging of Schwann Cell Development in Zebrafish
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    Chapter 28 Transplantation of Adult Rat Schwann Cells into the Injured Spinal Cord
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    Chapter 29 Schwann Cell Transplantation Methods Using Biomaterials
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    Chapter 30 Viral Transduction of Schwann Cells for Peripheral Nerve Repair
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    Chapter 31 Intraspinal Delivery of Schwann Cells for Spinal Cord Injury
Attention for Chapter 19: Purification of Exosomes from Primary Schwann Cells, RNA Extraction, and Next-Generation Sequencing of Exosomal RNAs
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About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • Good Attention Score compared to outputs of the same age (79th percentile)
  • High Attention Score compared to outputs of the same age and source (94th percentile)

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Chapter title
Purification of Exosomes from Primary Schwann Cells, RNA Extraction, and Next-Generation Sequencing of Exosomal RNAs
Chapter number 19
Book title
Schwann Cells
Published in
Methods in molecular biology, January 2018
DOI 10.1007/978-1-4939-7649-2_19
Pubmed ID
Book ISBNs
978-1-4939-7648-5, 978-1-4939-7649-2
Authors

Cristian De Gregorio, Paula Díaz, Rodrigo López-Leal, Patricio Manque, Felipe A. Court, Gregorio, Cristian De, Díaz, Paula, López-Leal, Rodrigo, Manque, Patricio, Court, Felipe A.

Abstract

Exosomes are small (30-150 nm) vesicles of endosomal origin secreted by most cell types. Exosomes contain proteins, lipids, and RNA species including microRNA, mRNA, rRNA, and long noncoding RNAs. The mechanisms associated with exosome synthesis and cargo loading are still poorly understood. A role for exosomes in intercellular communication has been reported in physiological and pathological conditions both in vitro and in vivo. Previous studies have suggested that Schwann cell-derived exosomes regulate neuronal functions, but the mechanisms are still unclear. Here, we describe protocols to establish rat neonatal Schwann cell cultures and to isolate exosomes from the conditioned medium of these cultures by differential ultracentrifugation. To analyze the RNA content of Schwann cell-derived exosomes, we detail protocols for RNA extraction and next-generation sequencing using miRNA and mRNA libraries. The protocol also includes RNA sequencing of Schwann cells, which allows the comparison between RNA content from cells and the secreted exosomes. Identification of RNAs present in Schwann cell-derived exosomes is a valuable tool to understand novel roles of Schwann cells in neuronal function in health and disease.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 35 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 35 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 7 20%
Professor 4 11%
Student > Ph. D. Student 3 9%
Student > Bachelor 2 6%
Student > Doctoral Student 1 3%
Other 2 6%
Unknown 16 46%
Readers by discipline Count As %
Neuroscience 6 17%
Agricultural and Biological Sciences 3 9%
Biochemistry, Genetics and Molecular Biology 3 9%
Unspecified 1 3%
Chemical Engineering 1 3%
Other 4 11%
Unknown 17 49%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 8. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 16 April 2018.
All research outputs
#4,035,354
of 23,043,346 outputs
Outputs from Methods in molecular biology
#1,039
of 13,194 outputs
Outputs of similar age
#88,028
of 442,409 outputs
Outputs of similar age from Methods in molecular biology
#87
of 1,499 outputs
Altmetric has tracked 23,043,346 research outputs across all sources so far. Compared to these this one has done well and is in the 82nd percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 13,194 research outputs from this source. They receive a mean Attention Score of 3.4. This one has done particularly well, scoring higher than 92% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 442,409 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 79% of its contemporaries.
We're also able to compare this research output to 1,499 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 94% of its contemporaries.