| Chapter title |
Gene Transfer and Gene Silencing in Stem Cells to Promote Chondrogenesis
|
|---|---|
| Chapter number | 7 |
| Book title |
Cartilage Tissue Engineering
|
| Published in |
Methods in molecular biology, January 2015
|
| DOI | 10.1007/978-1-4939-2938-2_7 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-2937-5, 978-1-4939-2938-2
|
| Authors |
Feng Zhang, Dong-An Wang |
| Abstract |
In stem cell-based chondrogenesis for articular cartilage regeneration, TGF-β3 is dosed to the stem cells to drive differentiation into chondrocytic cells. Meanwhile, type I collagen, which is endogenously expressed in some stem cells (e.g., synovium-derived mesenchymal stem cells) and upregulated by TGF-β3, poses a threat to chondrogenesis, as type I collagen may alter the components and stiffness of articular cartilage. Therefore, a wiser strategy would be to feed the cells with TGF-β3 while at the same time silencing the expression of type I collagen. In this chapter, methods for construction of adenoviral vectors and lentiviral vectors having both of the above functions are given. Their transduction into synovium-derived mesenchymal stem cells for articular cartilage engineering and following characterizations are also described. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 8 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 2 | 25% |
| Unspecified | 1 | 13% |
| Student > Bachelor | 1 | 13% |
| Student > Doctoral Student | 1 | 13% |
| Student > Master | 1 | 13% |
| Other | 0 | 0% |
| Unknown | 2 | 25% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 2 | 25% |
| Pharmacology, Toxicology and Pharmaceutical Science | 1 | 13% |
| Unspecified | 1 | 13% |
| Social Sciences | 1 | 13% |
| Medicine and Dentistry | 1 | 13% |
| Other | 0 | 0% |
| Unknown | 2 | 25% |