| Chapter title |
Decellularized Extracellular Matrix Scaffolds for Cartilage Regeneration
|
|---|---|
| Chapter number | 9 |
| Book title |
Cartilage Tissue Engineering
|
| Published in |
Methods in molecular biology, January 2015
|
| DOI | 10.1007/978-1-4939-2938-2_9 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-2937-5, 978-1-4939-2938-2
|
| Authors |
Shraddha Thakkar, Hugo Fernandes, Lorenzo Moroni |
| Abstract |
Decellularized extracellular matrix (ECM) is gaining a lot of attention as a biomaterial for tissue engineering applications. This chapter describes the processing techniques for decellularization of cell-derived ECM and protocols for the fabrication of ECM-based scaffolds in the form of hydrogels or fibrous polymer meshes by electrospinning. It describes the protocols to analyze the morphology and presence of collagen in fabricated scaffolds using scanning electron microscope and Picrosirius Red staining respectively. Methods to evaluate the metabolic activity and proliferation of cells (resazurin-based assay and DNA assay, respectively) and gene expression are also presented. Furthermore, histological techniques to analyze the presence of sulfated glycosaminoglycans are also described. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Turkey | 1 | 3% |
| Brazil | 1 | 3% |
| Unknown | 28 | 93% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 5 | 17% |
| Student > Doctoral Student | 3 | 10% |
| Other | 3 | 10% |
| Student > Bachelor | 3 | 10% |
| Professor | 3 | 10% |
| Other | 6 | 20% |
| Unknown | 7 | 23% |
| Readers by discipline | Count | As % |
|---|---|---|
| Medicine and Dentistry | 7 | 23% |
| Biochemistry, Genetics and Molecular Biology | 5 | 17% |
| Agricultural and Biological Sciences | 4 | 13% |
| Immunology and Microbiology | 1 | 3% |
| Psychology | 1 | 3% |
| Other | 3 | 10% |
| Unknown | 9 | 30% |