Chapter title |
An Integrated and Semiautomated Microscaled Approach to Profile Cis-Regulatory Elements by Histone Modification ChIP-Seq for Large-Scale Epigenetic Studies
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Book title |
Type 2 Immunity
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Published in |
Methods in molecular biology, January 2018
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DOI | 10.1007/978-1-4939-7896-0_22 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7895-3, 978-1-4939-7896-0
|
Authors |
Diana Youhanna Jankeel, Justin Cayford, Benjamin Joachim Schmiedel, Pandurangan Vijayanand, Grégory Seumois |
Abstract |
Chromatin immunoprecipitation followed by sequencing (ChIP-Seq) is the preferred approach to map histone modifications and identify cis-regulatory DNA elements throughout the genome. Multiple methods have been described to increase the efficiency of library preparation and to reduce hands-on time as well as costs. This review describes detailed steps to perform cell fixation, chromatin shearing, immunoprecipitation, and sequencing library preparation for a batch of 48-96 samples with small cell numbers. The protocol implements a semiautomated platform to reduce technical variability and improve signal-to-noise ratio as well as reduce hands-on time, thus allowing large-scale epigenetic studies of clinical samples with limited cell numbers. |
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Unknown | 1 | 100% |
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Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 8 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Master | 2 | 25% |
Student > Ph. D. Student | 1 | 13% |
Professor | 1 | 13% |
Other | 1 | 13% |
Student > Bachelor | 1 | 13% |
Other | 0 | 0% |
Unknown | 2 | 25% |
Readers by discipline | Count | As % |
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Immunology and Microbiology | 1 | 13% |
Medicine and Dentistry | 1 | 13% |
Unknown | 1 | 13% |