Chapter title |
The Generation and Use of Allergen-Specific TCR Transgenic Animals
|
---|---|
Chapter number | 15 |
Book title |
Type 2 Immunity
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-7896-0_15 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7895-3, 978-1-4939-7896-0
|
Authors |
Manon Vanheerswynghels, Wendy Toussaint, Martijn Schuijs, Leen Vanhoutte, Nigel Killeen, Hamida Hammad, Bart N. Lambrecht, Vanheerswynghels, Manon, Toussaint, Wendy, Schuijs, Martijn, Vanhoutte, Leen, Killeen, Nigel, Hammad, Hamida, Lambrecht, Bart N. |
Abstract |
The generation of allergen-specific TCR transgenic animals allows for the characterization of allergen-specific T-cell responses in vivo and in vitro and is a powerful tool to study adaptive immunity to allergens. Here we describe an approach starting from the isolation of antigen-specific T-cell hybridomas and using PCR, flow cytometric, and co-culture methods to obtain antigen-specific MHC class II-restricted CD4+ TCR transgenic mice on the Rag2-/- background. |
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Mendeley readers
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Unknown | 5 | 100% |
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Student > Ph. D. Student | 1 | 20% |
Student > Bachelor | 1 | 20% |
Researcher | 1 | 20% |
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Immunology and Microbiology | 1 | 20% |
Medicine and Dentistry | 1 | 20% |
Unknown | 3 | 60% |