Article
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Chapter title |
Imaging Precision-Cut Lung Slices to Visualize Leukocyte Localization and Trafficking
|
---|---|
Chapter number | 18 |
Book title |
Type 2 Immunity
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Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-7896-0_18 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7895-3, 978-1-4939-7896-0
|
Authors |
Miranda R. Lyons-Cohen, Hideki Nakano, Seddon Y. Thomas, Donald N. Cook |
Abstract |
Pulmonary dendritic cells (DCs) are potent antigen-presenting cells that can activate both naïve and memory/effector T cells. However, very little is known of how movements and localization of DCs contribute to these events. To study this, we have developed new procedures that combine precision-cut lung slices with cell staining using fluorescently tagged antibodies to detect individual cell types. In this chapter, we describe these methods in detail and show how they can be used to study the localization of not only DCs but also other leukocytes of interest, as well as structural cells within the lung. |
X Demographics
The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 1 | 50% |
United Kingdom | 1 | 50% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 1 | 50% |
Science communicators (journalists, bloggers, editors) | 1 | 50% |
Mendeley readers
The data shown below were compiled from readership statistics for 14 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 14 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Bachelor | 3 | 21% |
Student > Ph. D. Student | 3 | 21% |
Other | 1 | 7% |
Student > Postgraduate | 1 | 7% |
Student > Doctoral Student | 1 | 7% |
Other | 0 | 0% |
Unknown | 5 | 36% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 4 | 29% |
Immunology and Microbiology | 3 | 21% |
Medicine and Dentistry | 1 | 7% |
Unknown | 6 | 43% |
Attention Score in Context
This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 03 June 2021.
All research outputs
#13,620,235
of 23,092,602 outputs
Outputs from Methods in molecular biology
#3,668
of 13,207 outputs
Outputs of similar age
#220,383
of 442,643 outputs
Outputs of similar age from Methods in molecular biology
#352
of 1,499 outputs
Altmetric has tracked 23,092,602 research outputs across all sources so far. This one is in the 39th percentile – i.e., 39% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,207 research outputs from this source. They receive a mean Attention Score of 3.4. This one has gotten more attention than average, scoring higher than 70% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 442,643 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 48th percentile – i.e., 48% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 1,499 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 74% of its contemporaries.