Chapter title |
In vivo transfection by hepatitis A virus synthetic RNA.
|
---|---|
Chapter number | 20 |
Book title |
Positive-Strand RNA Viruses
|
Published in |
Archives of virology Supplementum, January 1994
|
DOI | 10.1007/978-3-7091-9326-6_20 |
Pubmed ID | |
Book ISBNs |
978-3-21-182522-8, 978-3-70-919326-6
|
Authors |
Emerson, S U, Lewis, M, Govindarajan, S, Shapiro, M, Moskal, T, Purcell, R H, Emerson, S. U., Lewis, M., Govindarajan, S., Shapiro, M., Moskal, T., Purcell, R. H. |
Abstract |
Marmosets injected intrahepatically with nucleic acids (cDNA and RNA transcripts) representing the full-length genome of the wild-type HM-175 strain of hepatitis A virus experienced acute hepatitis and seroconversion to hepatitis A virus capsid proteins. The hepatitis was comparable in severity to that caused by infection with the wild-type virus. The viral cDNA and the hepatitis A virus recovered from the feces of an injected animal contained the same marker mutation. Therefore, intermediate cell culture steps can be omitted and the virulence of a hepatitis A virus encoded by a cDNA clone can be evaluated by direct transfection of marmosets. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 3 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 1 | 33% |
Researcher | 1 | 33% |
Student > Postgraduate | 1 | 33% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 2 | 67% |
Social Sciences | 1 | 33% |