In vivo transfection by hepatitis A virus synthetic RNA.
Positive-Strand RNA Viruses
Archives of virology Supplementum, January 1994
Emerson, S U, Lewis, M, Govindarajan, S, Shapiro, M, Moskal, T, Purcell, R H, Emerson, S. U., Lewis, M., Govindarajan, S., Shapiro, M., Moskal, T., Purcell, R. H.
Marmosets injected intrahepatically with nucleic acids (cDNA and RNA transcripts) representing the full-length genome of the wild-type HM-175 strain of hepatitis A virus experienced acute hepatitis and seroconversion to hepatitis A virus capsid proteins. The hepatitis was comparable in severity to that caused by infection with the wild-type virus. The viral cDNA and the hepatitis A virus recovered from the feces of an injected animal contained the same marker mutation. Therefore, intermediate cell culture steps can be omitted and the virulence of a hepatitis A virus encoded by a cDNA clone can be evaluated by direct transfection of marmosets.
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