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Chapter title |
Immunofluorescence and Subsequent Confocal Microscopy of Intracellular TNF in Human Neutrophils.
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Chapter number | 24 |
Book title |
Cytokine Bioassays
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Published in |
Methods in molecular biology, June 2014
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DOI | 10.1007/978-1-4939-0928-5_24 |
Pubmed ID | |
Book ISBNs |
978-1-4939-0927-8, 978-1-4939-0928-5
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Authors |
Rollins J, Miskolci V, Janet Rollins, Veronika Miskolci |
Abstract |
Immunofluorescence is an important technique required to observe expression, localization and colocalization of proteins within the cell. Here we describe the immunofluorescence and subsequent confocal microscopy technique of tumor necrosis factor-α (TNF) in human neutrophils (polymorphonuclear leukocytes; PMN). The qualitative technique can be used to observe the expression pattern changes from resting to stimulated leukocytes. Colocalization with other cytokines, proteins, or organelles can be observed. This immunofluorescence technique can be done in 1-2 days. |
Mendeley readers
The data shown below were compiled from readership statistics for 3 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 3 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Bachelor | 1 | 33% |
Researcher | 1 | 33% |
Unknown | 1 | 33% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 2 | 67% |
Unknown | 1 | 33% |