Chapter title |
Cytokine detection by flow cytometry.
|
---|---|
Chapter number | 21 |
Book title |
Cytokine Bioassays
|
Published in |
Methods in molecular biology, June 2014
|
DOI | 10.1007/978-1-4939-0928-5_21 |
Pubmed ID | |
Book ISBNs |
978-1-4939-0927-8, 978-1-4939-0928-5
|
Authors |
Qiu JG, Mei XL, Chen ZS, Shi Z, Jian-Ge Qiu, Xiao-Long Mei, Zhe-Sheng Chen, Zhi Shi |
Abstract |
Analysis of intracellular cytokines is extremely important in the clinical treatment of numerous diseases. Flow cytometry (FCM) is a highly effective technique that detects intracellular cytokines using specific fluorescence-labeled antibodies. The common steps of this assay include cell collection, fixation, permeabilization, blocking, intracellular staining and analysis by FCM. This technique also allows for analyzing the biological function of cytokines. In this chapter, we describe a modified method to detect the specific intracellular cytokine staining using FCM, with an emphasis on the effects of variables including samples, temperature, buffers, data acquisition, and analysis. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 1 | 100% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Canada | 1 | 2% |
Unknown | 40 | 98% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Master | 6 | 15% |
Student > Bachelor | 6 | 15% |
Student > Ph. D. Student | 5 | 12% |
Student > Postgraduate | 3 | 7% |
Researcher | 2 | 5% |
Other | 4 | 10% |
Unknown | 15 | 37% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 6 | 15% |
Immunology and Microbiology | 4 | 10% |
Medicine and Dentistry | 4 | 10% |
Pharmacology, Toxicology and Pharmaceutical Science | 3 | 7% |
Agricultural and Biological Sciences | 2 | 5% |
Other | 6 | 15% |
Unknown | 16 | 39% |